Sources Of Error In Gel Filtration Chromatography
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Course Hero, Inc. Leaving solvent at the top of the column immediately before adding the solution to be filtered. Cytochrome C has a much higher pI value than BSA and it stay (+) charged when the phosphate buffer is being used. This preview shows document pages 5 - 6.
This is the end of the preview. Title: Affinity Chromatography Obje Experiment 6 Erik, Odom Page5 / 6 The sources of error in this experiment could have come... Trending Could humans evolve into zebras? 14 answers How do we know science is right? 31 answers It's been many years since Darwin claimed Evolution is real but we still haven't Please upload a file larger than 100x100 pixels We are experiencing some problems, please try again.
Log in Sign up Home Baylor BIO BIO 4141 Experiment write-up #2 The sources of error in this experiment could have SCHOOL Baylor COURSE TITLE BIO 4141 TYPE Notes UPLOADED BY Would you be able to separate catalase and haemoglobin by the technique of gel-filtration? Sign up to access the rest of the document. You can only upload photos smaller than 5 MB.
Course Hero is not sponsored or endorsed by any college or university. The 2M Tris, at a pH of 8.00, acting as a salt, caused the cytochrome C to not interact and therefore elute while the BSA stayed in the column (or if If there is already solution on top of the beads, and you have not allowed it to drain so that the top of the beads are exposed to air, the solution View Full Document Company About Us Scholarships Sitemap Standardized Tests Get Course Hero iOS Android Educators Careers Our Team Jobs Internship Help Contact Us FAQ Feedback Legal Copyright Policy Honor Code
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What does "A disk read error occurred" means? Report this document Report View Full Document Most Popular Documents for BIO 4141 2 pages Calcium complex Baylor BIO 4141 - Spring 2013 Erik Odom CHE 2216 11/15/2012 Complexometric Determination of Why tubulin heterodimer has a GTP bindingsite While actin has an ATP binding site? If you under pack the column, there is not enough bead surface area to interact with all of the smaller particles.
Sign up to view the full content. check over here The phosphate buffer’s pH was 7.00. Ask a homework question - tutors are online Difference between natural and artificial media in animal tissue culture?
Both result in smaller particles moving too quickly through the column. 2. Cory · 6 years ago 0 Thumbs up 0 Thumbs down Comment Add a comment Submit · just now Report Abuse Add your answer Where are errors most likely to occur View Full Document The separation of BSA from cytochrome C is possible due to a difference in the pI for each of the different compounds. his comment is here How would I use the scientific method for an experiment of how temperature affects plant growth?
Hopefully those make sense Source(s): Factors affecting filtration: http://en.wikipedia.org/wiki/Size-exclus... ⌂HomeMailSearchNewsSportsFinanceCelebrityWeatherAnswersFlickrMobileMore⋁PoliticsMoviesMusicTVGroupsStyleBeautyTechShoppingInstall the new Firefox» Yahoo Answers 👤 Sign in ✉ Mail ⚙ Help Account Info Help Suggestions Send Feedback Answers Home All Categories Arts & Humanities Beauty & Style Business & You can only upload a photo or a video.
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More questions What situation has to occur for you to say that person needs to be whacked up side the head with a 2 by 4? Health Sciences “Ion Exchange Chromatography, Principles, and Methods” Handbook. • Sigma catalog information on CM-sepharose and the components of the mixture (BSA and cytochrome c). • Technical Information for CM-sepharose from The sources of error in this experiment could have come from incorrectly labeling the 2 mL mark on the test tubes, not cleaning the glass cuvette well, disturbing the column, or Ion Exchange: This preview has intentionally blurred sections.
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