Sources Of Error When Growing Bacteria
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Be sure to label each petri dish with a name or number. Q: Does salt affect the freezing point of water? Thanks for your consideration Add your answer Source Submit Cancel Report Abuse I think this question violates the Community Guidelines Chat or rant, adult content, spam, insulting other members,show more I It was seen that the bacteria without the plasmid produced a lawn in normal conditions, but grew only in colonies in the Ampicillin condition.
Pour off excess iodine and rinse the slide with water as before. 6. Possible sources of error in coefficient of linear expansion Lab? Eubacteria are placed in a division depending on the type of cell wall they have. Let it cool then remove a small amount of culture from the agar surface; touch it several times to the drop of water until it just turns cloudy.
ARA is the L-arabinose operon for E.coli and you must know this to understand your lab. The second was laced with ampicillin, and would be used with bacteria that did not receive the plasmid. Depending on bacteria abundance on the swab, quadrant 4 may show no grow or only a few colonies. NC-7335 Alcohol Burner.
Bacteriology – Lab Report Guidelines Must be TYPED. Human error is another potential source of error. It can be concluded that the GFP could only be expressed when arabinose was present in the environment. This keeps condensation which forms in the lid from dropping onto and disrupting the bacteria growing surface.
In this type of project, a petri dish is inoculated with bacteria then a paper disk (filter paper) treated with an antiseptic agent is placed in the dish. Contaminants may react with one of the reactants, leading to a decrease in the desired reaction. The system returned: (22) Invalid argument The remote host or network may be down. This hypothesis was supported in this lab.
With bacteria from your fingers or falling out of your hair? The third plate was the same as the second, except only bacteria with the plasmid would be grown on it. Offline Stupidity Kills Outside, huh?********** Posts: 4,501 Join Date: December 19th 2009 Re: Biology Help - May 24th 2010, 11:39 PM Before explaining it, I'll say what the things I understood that he said that putting it in the water bath with make the cell membrane permeable, but what is the point of putting it in ice, water bath, back
There were many potential sources of error in this lab. check over here Terms Colony Shape and size -- round, irregular, punctiform (tiny) Margin edge -- entire (smooth), undulate (wavy), lobate (lobed) Elevation -- convex, flat, raised Color -- color + opaque, translucent, shiny NC-1319 © The Science Company Site Map Quick Order Wishlist Gift Ideas Return Policy Customer Account Log in (Register | Forgotten password) Home Get Advice About About Mission Statement Project Blog Sources of error depend on the exact method of synthesis.
If done correctly, you should be able to identify your bacteria as Gram + or Gram - and check resouces to see if you are correct. Another common technique is to divide each plate into four quadrants by marking the lid with a cross. Scientists have found that it is possible to integrate foreign DNA into bacteria by inserting a pre-constructed, circular, double-stranded DNA called a plasmid. http://nzbsites.com/sources-of/sources-of-error-in-hplc.html In the pGLO plasmid, the arabinose operon codes for GFP when in the presence of arabinose.
Contamination of agar plate? I don't really know what we are trying to find or do. The Science Company has these available at this link.
I can't find MY lab report, but I have my friends on my computer...
- The other two conditions included the integration of the plasmid in the bacteria cells, and consisted of a dish with Ampicillin and one with Ampicillin and arabinose sugar.
- This is the area for your own reflections and thoughts.
- Prepare sterilized water by boiling water and letting cool to room temperature.
Plasmids replicate independently from the bacterial chromosomes, and are especially important when bacteria have to evolve to specific environmental hindrances. In this case, bacteria became resistant to an antibiotic--a chemical that is supposed to induce reproduction. Collect bacteria from each location using one swab for each new spot. When you return the cells to the ice bath, you decrease the activity of the channels and prevent the heat shock from causing too much damage.
I believe that varying the temperature is probably to restrict some of the growth of the E.coli colony and may degenerate some of the proteins so as to make the effects Generated Sat, 29 Oct 2016 02:13:53 GMT by s_fl369 (squid/3.5.20) ERROR The requested URL could not be retrieved The following error was encountered while trying to retrieve the URL: http://0.0.0.10/ Please try the request again. weblink This is because the arabinose operon can only express GFP if arabinose binds to its receptors.
Introduction: Escherichia coli (E.Coli) is a bacteria that primarily lives in the lower intestine of human beings, but is also one of the most commonly found strains of bacteria in the Search engine optimization by vBSEO. Without tearing the agar surface, inoculate the dish by gently pressing fingers, finger nails, coin, etc onto agar surface. (Direct contact of lips or tongue is NOT a good idea.) Replace Ask in this forum if you need help with coursework, applications, and more.
Sterile swabs or inoculating loop. Alcohol burner (source of flame to sterilize inoculating loop). When you return the cells to the ice bath, you decrease the activity of the channels and prevent the heat shock from causing too much damage. You may need to repeat this process a few times in order to get a good slide! *Identify the shape and arrangement of the bacteria.
Prepared petri dishes containing agar medium and nutrients. Let us know what you tested and how your experiment turned out. Inoculate each dish by streaking a pattern gently across the entire agar surface without tearing into it. Or keeping the agar plates open (uncovered) for long time and thereby collect samples from the air?
Terms | Legal | Privacy | Conduct Powered by vBulletin.Copyright ©2000-2016, Jelsoft Enterprises Ltd. Bacteria collected from doorknobs, bathroom fixtures, etc. User Name Remember Me? Now, inoculate a petri dish by pouring the water into the dish so the entire surface is covered.
cAMP is used for tons of things, such as forming ATP, which can later be broken into ADP + Pi (inorganic phosphate) to release energy. The dishes will start to smell which means the bacteria are growing. Bleach. Examine the slide under the microscope using the high power objective.
Experiment 1: Direct Contact Discussion. I'm sure you're going to do this or your teacher will explain it but one way is to use CaCl2.
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